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transcriptor reverse transcriptase  (Roche)


Bioz Manufacturer Symbol Roche manufactures this product  
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    Structured Review

    Roche transcriptor reverse transcriptase
    Transcriptor Reverse Transcriptase, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptor+reverse+transcriptase/pm39071953-239-9-12
    Average 86 stars, based on 1 article reviews
    transcriptor reverse transcriptase - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: TNF-α synergises with IFN-γ to induce caspase-8-JAK1/2-STAT1-dependent death of intestinal epithelial cells.
    Article Snippet: Biopsy homogenates were processed using the RNeasy Mini kit and RNA Clean & Concentrator-5 kit (R1013, Zymo Research). .. Synthesis of cDNA was performed on 500 ng of RNA from cell lines (or 200 ng from biopsies) using Transcriptor Reverse Transcriptase (3531287001, Roche) and random hexamers. .. Gene expression analysis was done using Universal ProbeLibrary System (Roche) and Sensi Mix II Probe Kit reagent (BIO-83005, Bioline) according to the manufacturer’s instructions.

    Article Title: The high concentrations of abscisic, jasmonic, and salicylic acids produced under long days do not accelerate flowering in Chenopodium ficifolium 459.
    Article Snippet: RNA concentration and quality were checked on 0.9% agarose gel and using the NanoDrop (Thermo Fisher Scientific, Vantaa, Finland). .. One μg of RNA and oligo dT primers (500 ng) were heated for 5 min at 65 ◦C, chilled on ice and mixed with Transcriptor buffer (Roche), 0.5 μl of Protector RNase Inhibitor (Roche, Diagnostics, Mannheim, Germany), 2 μl of 10 mM dNTPs and 10 units of Transcriptor Reverse Transcriptase (Roche). .. The first strand of cDNA was produced at 55 ◦C for 30 min. Two technical replicates of cDNA were produced with each RNA specimen. qPCR was performed using the LightCycler 480 SYBR Green I Master (Roche) in a final volume of 10 μl with 500 nM of each of the primers.

    Article Title: Transcriptomic study of the night break in Chenopodium rubrum reveals possible upstream regulators of the floral activator CrFTL1.
    Article Snippet: The transition from vegetative to reproductive phases is the most fundamental and tightly controlled switch in the life of flowering plants.. The short-day plant Chenopodium rubrum is a fast cycling annual plant lacking a juvenile phase.. It can be induced to flowering at the seedling stage by exposure to a single period of darkness.

    Article Title: Differential translation of mRNA isoforms underlies oncogenic activation of cell cycle kinase Aurora A
    Article Snippet: Aliquots of each fraction were mixed 1:1 with NuPAGE LDS Sample Buffer 4X (NP0007, Invitrogen) and 10 mM DTT (10197777001, Sigma), boiled 3 min at 95°C and stored at –20°C. .. cDNA synthesis was performed using the Transcriptor Reverse Transcriptase (3531317001, Roche) with an oligo-dT anchor primer (5′- GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTT V-3′). ..

    Article Title: APOBEC3 degradation is the primary function of HIV-1 Vif for virus replication in the myeloid cell line THP-1
    Article Snippet: Total RNA was isolated from THP-1 by RNA Premium Kit (NIPPON Genetics, Cat# FG-81250). .. Then, cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) and used to amplify A3C or A3H gene with the following primers [ A3C outer primers: (5’-GCG CTT CAG AAA AGA GTG GG) and (5’-GGA GAC AGA CCA TGA GGC). ..

    Article Title: Leukolectins support lifelong innate immunity in lower vertebrates, and reveal dichotomies of several leukocytic lineages.
    Article Snippet: Innate immunity is vital for animal homeostasis and survival.. First-line immuno-defense for fish larvae involves mucus enriched with leukolectin (LL) secreted by dermal lectocytes.. Later during the critical transition from yolk-nutrition to feeding, additional larval immuno-protection in zebrafish (zF) is provided by macrophages containing LL (lectophages).

    Article Title: Functional benefit of CRISPR-Cas9-induced allele deletion for RYR1 dominant mutation.
    Article Snippet: RNA has been extracted from myotubes differentiated for 7 days using Trizol (Life Technologies) followed by chloroform/isoamyl alcohol (49:1 v/v). .. RNA has been retro-transcribed to cDNA using oligo-dT and transcriptor reverse transcriptase (Roche) according to the manufacturer’s recommendations. .. PCR amplification of the DNA has been performed with Phusion high-fidelity master mix with GC buffer (ref F532L, ThermoFisher).

    Article Title: Human Microglia-Like Cells Differentiated from Monocytes with GM-CSF and IL-34 Show Phagocytosis of α-Synuclein Aggregates and C/EBPβ-Dependent Proinflammatory Activation.
    Article Snippet: .. RNA was reverse transcribed with random primers using Transcriptor Reverse Transcriptase (Roche Diagnostics) to obtain cDNA by using qScriptTM cDNA Synthesis Kit (Quanta Biosciences, Ref. 95,047) according to manufacturer’s instructions. ..

    cDNA Synthesis:

    Article Title: Differential translation of mRNA isoforms underlies oncogenic activation of cell cycle kinase Aurora A
    Article Snippet: Aliquots of each fraction were mixed 1:1 with NuPAGE LDS Sample Buffer 4X (NP0007, Invitrogen) and 10 mM DTT (10197777001, Sigma), boiled 3 min at 95°C and stored at –20°C. .. cDNA synthesis was performed using the Transcriptor Reverse Transcriptase (3531317001, Roche) with an oligo-dT anchor primer (5′- GACCACGCGTATCGATGTCGACTTTTTTTTTTTTTTTT V-3′). ..

    Article Title: Human Microglia-Like Cells Differentiated from Monocytes with GM-CSF and IL-34 Show Phagocytosis of α-Synuclein Aggregates and C/EBPβ-Dependent Proinflammatory Activation.
    Article Snippet: .. RNA was reverse transcribed with random primers using Transcriptor Reverse Transcriptase (Roche Diagnostics) to obtain cDNA by using qScriptTM cDNA Synthesis Kit (Quanta Biosciences, Ref. 95,047) according to manufacturer’s instructions. ..

    Synthesized:

    Article Title: APOBEC3 degradation is the primary function of HIV-1 Vif for virus replication in the myeloid cell line THP-1
    Article Snippet: Total RNA was isolated from THP-1 by RNA Premium Kit (NIPPON Genetics, Cat# FG-81250). .. Then, cDNA was synthesized by Transcriptor Reverse Transcriptase (Roche, Cat# 03531287001) and used to amplify A3C or A3H gene with the following primers [ A3C outer primers: (5’-GCG CTT CAG AAA AGA GTG GG) and (5’-GGA GAC AGA CCA TGA GGC). ..

    Purification:

    Article Title: Leukolectins support lifelong innate immunity in lower vertebrates, and reveal dichotomies of several leukocytic lineages.
    Article Snippet: Innate immunity is vital for animal homeostasis and survival.. First-line immuno-defense for fish larvae involves mucus enriched with leukolectin (LL) secreted by dermal lectocytes.. Later during the critical transition from yolk-nutrition to feeding, additional larval immuno-protection in zebrafish (zF) is provided by macrophages containing LL (lectophages).



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